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Serum mitochondrial tsRNA serves as a novel biomarker for hepatocarcinoma diagnosis
《医学前沿(英文)》 2022年 第16卷 第2期 页码 216-226 doi: 10.1007/s11684-022-0920-7
Hongying ZHANG, Jianwu TANG, Wenting ZHU, Chunxiu HU, Guowang XU
《医学前沿(英文)》 2009年 第3卷 第2期 页码 119-129 doi: 10.1007/s11684-009-0022-9
关键词: murine ascites hepatocarcinoma cell line metastasis curcumin drug sensitivity
null
《医学前沿(英文)》 2017年 第11卷 第1期 页码 120-128 doi: 10.1007/s11684-017-0501-3
Norepinephrine transporter (NET) transfection leads to significant uptake of iodine-131-labeled metaiodobenzylguanidine (131I-MIBG) in non-neuroendocrine tumors. However, the use of 131I-MIBG is limited by its short retention time in target cells. To prolong the retention of 131I-MIBG in target cells, we infected hepatocarcinoma (HepG2) cells with Lentivirus-encoding human NET and vesicular monoamine transporter 2 (VMAT2) genes to obtain NET-expressing, NET-VMAT2-coexpressing, and negative-control cell lines. We evaluated the uptake and efflux of 131I-MIBG both in vitro and in vivo in mice bearing transfected tumors. NET-expressing and NET-VMAT2-coexpressing cells respectively showed 2.24 and 2.22 times higher 131I-MIBG uptake than controls. Two hours after removal of 131I-MIBG-containing medium, 25.4% efflux was observed in NET-VMAT2-coexpressing cells and 38.6% in NET-expressing cells. In vivo experiments were performed in nude mice bearing transfected tumors; results revealed that NET-VMAT2-coexpressing tumors had longer 131I-MIBG retention time than NET-expressing tumors. Meanwhile, NET-VMAT2-coexpressing and NET-expressing tumors displayed 0.54% and 0.19%, respectively, of the injected dose per gram of tissue 24 h after 131I-MIBG administration. Cotransfection of HepG2 cells with NET and VMAT2resulted in increased 131I-MIBG uptake and retention. However, the degree of increase was insufficient to be therapeutically effective in target cells.
关键词: norepinephrine transporter vesicular monoamine transporter 2 -MIBG gene therapy lentivirus vector
标题 作者 时间 类型 操作
Establishment and drug sensitivity evaluation of murine ascites hepatocarcinoma cell line with high lymphatic
Hongying ZHANG, Jianwu TANG, Wenting ZHU, Chunxiu HU, Guowang XU
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